Subcellular distribution of GLUT4 in Chinese hamster ovary cells overexpressing mutant dynamin: evidence that dynamin is a regulatory GTPase in GLUT4 endocytosis.
Identifieur interne : 000292 ( Main/Exploration ); précédent : 000291; suivant : 000293Subcellular distribution of GLUT4 in Chinese hamster ovary cells overexpressing mutant dynamin: evidence that dynamin is a regulatory GTPase in GLUT4 endocytosis.
Auteurs : W. Omata [Japon] ; H. Shibata ; Y. Suzuki ; S. Tanaka ; T. Suzuki ; K. Takata ; I. KojimaSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 1997.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Cellules CHO (MeSH), Compartimentation cellulaire (MeSH), Cricetinae (MeSH), Dynamines (MeSH), Endocytose (MeSH), Fragments peptidiques (génétique), Fragments peptidiques (métabolisme), Protéines du muscle (MeSH), Protéines recombinantes (métabolisme), Récepteur à l'insuline (métabolisme), Transport biologique (MeSH), Transporteur de glucose de type 4 (MeSH), Transporteurs de monosaccharides (isolement et purification), Transporteurs de monosaccharides (métabolisme), dGTPases (génétique), dGTPases (métabolisme).
- MESH :
- génétique : Fragments peptidiques, dGTPases.
- isolement et purification : Transporteurs de monosaccharides.
- métabolisme : Fragments peptidiques, Protéines recombinantes, Récepteur à l'insuline, Transporteurs de monosaccharides, dGTPases.
- Animaux, Cellules CHO, Compartimentation cellulaire, Cricetinae, Dynamines, Endocytose, Protéines du muscle, Transport biologique, Transporteur de glucose de type 4.
English descriptors
- KwdEn :
- Animals (MeSH), Biological Transport (MeSH), CHO Cells (MeSH), Cell Compartmentation (MeSH), Cricetinae (MeSH), Dynamins (MeSH), Endocytosis (MeSH), GTP Phosphohydrolases (genetics), GTP Phosphohydrolases (metabolism), Glucose Transporter Type 4 (MeSH), Monosaccharide Transport Proteins (isolation & purification), Monosaccharide Transport Proteins (metabolism), Muscle Proteins (MeSH), Peptide Fragments (genetics), Peptide Fragments (metabolism), Receptor, Insulin (metabolism), Recombinant Proteins (metabolism).
- MESH :
- chemical , genetics : GTP Phosphohydrolases, Peptide Fragments.
- chemical , isolation & purification : Monosaccharide Transport Proteins.
- chemical , metabolism : GTP Phosphohydrolases, Monosaccharide Transport Proteins, Peptide Fragments, Receptor, Insulin, Recombinant Proteins.
- chemical : Dynamins, Glucose Transporter Type 4, Muscle Proteins.
- Animals, Biological Transport, CHO Cells, Cell Compartmentation, Cricetinae, Endocytosis.
Abstract
We have investigated the subcellular distribution of GLUT4 by immunofluorescence microscopy after transfection with wild-type or mutant dynamin cDNA into Chinese hamster ovary cells expressing insulin receptor and GLUT4. In the basal state, GLUT4 was distributed exclusively within the cells in the cells overexpressing wild-type dynamin (CHOIR-GLUT4-WT) but was located at the cell surface in the cells overexpressing mutant dynamin (CHOIR-GLUT4-K44E). Insulin induced subcellular shift of GLUT4 to the cell surface in CHOIR-GLUT4-WT cells but had little effect in CHOIR-GLUT4-K44E cells. When insulin-stimulated cells were treated with wortmannin, GLUT4 was redistributed within the cells in CHOIR-GLUT4-WT cell, whereas it remained at the cell surface in CHOIR-GLUT4-K44E cell. These results suggest that dynamin is a regulatory GTPase in endocytosis of GLUT4.
DOI: 10.1006/bbrc.1997.7810
PubMed: 9425283
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Tanaka</name></author><author><name sortKey="Suzuki, T" sort="Suzuki, T" uniqKey="Suzuki T" first="T" last="Suzuki">T. Suzuki</name></author><author><name sortKey="Takata, K" sort="Takata, K" uniqKey="Takata K" first="K" last="Takata">K. Takata</name></author><author><name sortKey="Kojima, I" sort="Kojima, I" uniqKey="Kojima I" first="I" last="Kojima">I. Kojima</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1997">1997</date><idno type="RBID">pubmed:9425283</idno><idno type="pmid">9425283</idno><idno type="doi">10.1006/bbrc.1997.7810</idno><idno type="wicri:Area/Main/Corpus">000295</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000295</idno><idno type="wicri:Area/Main/Curation">000292</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000292</idno><idno type="wicri:Area/Main/Exploration">000292</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Subcellular distribution of GLUT4 in Chinese hamster ovary cells overexpressing mutant dynamin: evidence that dynamin is a regulatory GTPase in GLUT4 endocytosis.</title><author><name sortKey="Omata, W" sort="Omata, W" uniqKey="Omata W" first="W" last="Omata">W. Omata</name><affiliation wicri:level="1"><nlm:affiliation>Department of Cell Biology, Gunma University, Maebashi, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Cell Biology, Gunma University, Maebashi</wicri:regionArea><wicri:noRegion>Maebashi</wicri:noRegion></affiliation></author><author><name sortKey="Shibata, H" sort="Shibata, H" uniqKey="Shibata H" first="H" last="Shibata">H. Shibata</name></author><author><name sortKey="Suzuki, Y" sort="Suzuki, Y" uniqKey="Suzuki Y" first="Y" last="Suzuki">Y. Suzuki</name></author><author><name sortKey="Tanaka, S" sort="Tanaka, S" uniqKey="Tanaka S" first="S" last="Tanaka">S. Tanaka</name></author><author><name sortKey="Suzuki, T" sort="Suzuki, T" uniqKey="Suzuki T" first="T" last="Suzuki">T. Suzuki</name></author><author><name sortKey="Takata, K" sort="Takata, K" uniqKey="Takata K" first="K" last="Takata">K. Takata</name></author><author><name sortKey="Kojima, I" sort="Kojima, I" uniqKey="Kojima I" first="I" last="Kojima">I. Kojima</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="1997" type="published">1997</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Biological Transport (MeSH)</term><term>CHO Cells (MeSH)</term><term>Cell Compartmentation (MeSH)</term><term>Cricetinae (MeSH)</term><term>Dynamins (MeSH)</term><term>Endocytosis (MeSH)</term><term>GTP Phosphohydrolases (genetics)</term><term>GTP Phosphohydrolases (metabolism)</term><term>Glucose Transporter Type 4 (MeSH)</term><term>Monosaccharide Transport Proteins (isolation & purification)</term><term>Monosaccharide Transport Proteins (metabolism)</term><term>Muscle Proteins (MeSH)</term><term>Peptide Fragments (genetics)</term><term>Peptide Fragments (metabolism)</term><term>Receptor, Insulin (metabolism)</term><term>Recombinant Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Cellules CHO (MeSH)</term><term>Compartimentation cellulaire (MeSH)</term><term>Cricetinae (MeSH)</term><term>Dynamines (MeSH)</term><term>Endocytose (MeSH)</term><term>Fragments peptidiques (génétique)</term><term>Fragments peptidiques (métabolisme)</term><term>Protéines du muscle (MeSH)</term><term>Protéines recombinantes (métabolisme)</term><term>Récepteur à l'insuline (métabolisme)</term><term>Transport biologique (MeSH)</term><term>Transporteur de glucose de type 4 (MeSH)</term><term>Transporteurs de monosaccharides (isolement et purification)</term><term>Transporteurs de monosaccharides (métabolisme)</term><term>dGTPases (génétique)</term><term>dGTPases (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>GTP Phosphohydrolases</term><term>Peptide Fragments</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Monosaccharide Transport Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>GTP Phosphohydrolases</term><term>Monosaccharide Transport Proteins</term><term>Peptide Fragments</term><term>Receptor, Insulin</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Dynamins</term><term>Glucose Transporter Type 4</term><term>Muscle Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Fragments peptidiques</term><term>dGTPases</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Transporteurs de monosaccharides</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Fragments peptidiques</term><term>Protéines recombinantes</term><term>Récepteur à l'insuline</term><term>Transporteurs de monosaccharides</term><term>dGTPases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Biological Transport</term><term>CHO Cells</term><term>Cell Compartmentation</term><term>Cricetinae</term><term>Endocytosis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules CHO</term><term>Compartimentation cellulaire</term><term>Cricetinae</term><term>Dynamines</term><term>Endocytose</term><term>Protéines du muscle</term><term>Transport biologique</term><term>Transporteur de glucose de type 4</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have investigated the subcellular distribution of GLUT4 by immunofluorescence microscopy after transfection with wild-type or mutant dynamin cDNA into Chinese hamster ovary cells expressing insulin receptor and GLUT4. In the basal state, GLUT4 was distributed exclusively within the cells in the cells overexpressing wild-type dynamin (CHOIR-GLUT4-WT) but was located at the cell surface in the cells overexpressing mutant dynamin (CHOIR-GLUT4-K44E). Insulin induced subcellular shift of GLUT4 to the cell surface in CHOIR-GLUT4-WT cells but had little effect in CHOIR-GLUT4-K44E cells. When insulin-stimulated cells were treated with wortmannin, GLUT4 was redistributed within the cells in CHOIR-GLUT4-WT cell, whereas it remained at the cell surface in CHOIR-GLUT4-K44E cell. These results suggest that dynamin is a regulatory GTPase in endocytosis of GLUT4.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9425283</PMID><DateCompleted><Year>1998</Year><Month>01</Month><Day>26</Day></DateCompleted><DateRevised><Year>2009</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0006-291X</ISSN><JournalIssue CitedMedium="Print"><Volume>241</Volume><Issue>2</Issue><PubDate><Year>1997</Year><Month>Dec</Month><Day>18</Day></PubDate></JournalIssue><Title>Biochemical and biophysical research communications</Title></Journal><ArticleTitle>Subcellular distribution of GLUT4 in Chinese hamster ovary cells overexpressing mutant dynamin: evidence that dynamin is a regulatory GTPase in GLUT4 endocytosis.</ArticleTitle><Pagination><MedlinePgn>401-6</MedlinePgn></Pagination><Abstract><AbstractText>We have investigated the subcellular distribution of GLUT4 by immunofluorescence microscopy after transfection with wild-type or mutant dynamin cDNA into Chinese hamster ovary cells expressing insulin receptor and GLUT4. In the basal state, GLUT4 was distributed exclusively within the cells in the cells overexpressing wild-type dynamin (CHOIR-GLUT4-WT) but was located at the cell surface in the cells overexpressing mutant dynamin (CHOIR-GLUT4-K44E). Insulin induced subcellular shift of GLUT4 to the cell surface in CHOIR-GLUT4-WT cells but had little effect in CHOIR-GLUT4-K44E cells. When insulin-stimulated cells were treated with wortmannin, GLUT4 was redistributed within the cells in CHOIR-GLUT4-WT cell, whereas it remained at the cell surface in CHOIR-GLUT4-K44E cell. These results suggest that dynamin is a regulatory GTPase in endocytosis of GLUT4.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Omata</LastName><ForeName>W</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Cell Biology, Gunma University, Maebashi, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shibata</LastName><ForeName>H</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Suzuki</LastName><ForeName>Y</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Tanaka</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Suzuki</LastName><ForeName>T</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Takata</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Kojima</LastName><ForeName>I</ForeName><Initials>I</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biochem Biophys Res Commun</MedlineTA><NlmUniqueID>0372516</NlmUniqueID><ISSNLinking>0006-291X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D051275">Glucose Transporter Type 4</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009004">Monosaccharide Transport Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009124">Muscle Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010446">Peptide 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Compartmentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006224" MajorTopicYN="N">Cricetinae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D034281" MajorTopicYN="N">Dynamins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004705" MajorTopicYN="Y">Endocytosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020558" MajorTopicYN="N">GTP Phosphohydrolases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051275" MajorTopicYN="N">Glucose Transporter Type 4</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009004" MajorTopicYN="N">Monosaccharide Transport Proteins</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009124" MajorTopicYN="Y">Muscle Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010446" MajorTopicYN="N">Peptide Fragments</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011972" MajorTopicYN="N">Receptor, Insulin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1998</Year><Month>1</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1998</Year><Month>1</Month><Day>13</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1998</Year><Month>1</Month><Day>13</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9425283</ArticleId><ArticleId IdType="pii">S0006-291X(97)97810-4</ArticleId><ArticleId IdType="doi">10.1006/bbrc.1997.7810</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Kojima, I" sort="Kojima, I" uniqKey="Kojima I" first="I" last="Kojima">I. Kojima</name><name sortKey="Shibata, H" sort="Shibata, H" uniqKey="Shibata H" first="H" last="Shibata">H. Shibata</name><name sortKey="Suzuki, T" sort="Suzuki, T" uniqKey="Suzuki T" first="T" last="Suzuki">T. Suzuki</name><name sortKey="Suzuki, Y" sort="Suzuki, Y" uniqKey="Suzuki Y" first="Y" last="Suzuki">Y. Suzuki</name><name sortKey="Takata, K" sort="Takata, K" uniqKey="Takata K" first="K" last="Takata">K. Takata</name><name sortKey="Tanaka, S" sort="Tanaka, S" uniqKey="Tanaka S" first="S" last="Tanaka">S. Tanaka</name></noCountry><country name="Japon"><noRegion><name sortKey="Omata, W" sort="Omata, W" uniqKey="Omata W" first="W" last="Omata">W. Omata</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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